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31 May 2012
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A fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 (Cat. Nos. 208712, 208713, 208715, and 208718) based on 106 known cleavage sites. It is cleaved by mammalian calpains with a much better efficacy (kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively) and kinetically superior to other commonly used calpain substrates, such as LY-AMC (Cat. No. 208731) and α-spectrin cleavage site-based substrate (Cat. No. 208748). Has been used successfully in monitoring calpain activity in whole Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate.
| Product information | |||
|---|---|---|---|
| Peptide Sequence | DABCYL-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-EDANS | ||
| Form | Red lyophilized solid | ||
| Formulation | Supplied as a trifluoroacetate salt. | ||
| Molar mass | 1676 | ||
| Protect from Light | Yes | ||
| Packaged under inert gas | Yes | ||
| Purity | ≥98% by HPLC | ||
| Excitation max. | 320 nm | ||
| Emission max. | 480 nm | ||
| Solubility | DMSO or H2O | ||
| Chemical formula | C80H114N20O18S | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Irritant |
||
