For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
31 May 2012
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This kit provides a simple and convenient assay for measuring the activity of caspase-1 and related caspases that recognize the sequence YVAD. The assay is based on detection of the cleavage of the substrate YVAD-AFC. Upon cleavage of the substrate by caspase-1 or related caspases, free AFC emits a yellow-green fluorescence (~505 nm), which can be measured using a fluorometer or a fluorescence microplate reader.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 100 Tests | ||
| Detection method | Fluorometric | ||
| Protect from Light | Yes | ||
| Assay time | 3-3.5 h | ||
| Sample type | cell lysates | ||
| Kit contains | Cell Lysis Buffer, Reaction Buffer, YVAD-AFC Substrate, DTT, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Ambient Temperature Only
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 26-36-39-61 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. Wear eye/face protection. Avoid release to the environment. Refer to special instructions/safety data sheet. |
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| R Phrase |
R: 22-36/38-41-52/53 Harmful if swallowed. Irritating to eyes and skin. Risk of serious damage to eyes. Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment. |
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