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QIA107  Caspase-3 Immunoassay/Activity Kit, Fluorometric

For general questions please contact our Customer Service:

Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000

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31 May 2012

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Specifically detects caspase-3 activity using the DEVD-AFC substrate. Though caspase-3 substrates such as DEVD will also detect the activity of other caspases, this kit ensures specificity by first capturing caspase-3 using a selective antibody.
Substitutes
QIA70 Caspase-3 Activity Assay Kit, Fluorometric
Product information
Format 96-well plate
Form 96 Tests
Detection method Fluorometric
Species reactivity human, mouse, rat
Protect from Light Yes
Avoid freeze/thaw Yes
Assay time 6 h
Sample type Cultured cells
Kit contains Cell Lysis Buffer, Coating Buffer, Anti-Caspase-3 Antibody, Blocking Buffer, Incubation Buffer, DTT, DEVD-AFC Substrate, Recombinant Caspase-3, 96-Well Plate, Plate Sealer, and a user protocol.
Excitation max. 370-425 nm
Emission max. 490-525 nm
Store and ship information
Storage -20°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS
Safety information
S Phrase S: 24-26-39-61

Avoid contact with skin.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear eye/face protection.
Avoid release to the environment. Refer to special instructions/safety data sheet.
R Phrase R: 22-38-41-52/53

Harmful if swallowed.
Irritating to skin.
Risk of serious damage to eyes.
Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment.

© Merck KGaA, Darmstadt, Germany, 2012


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