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Merck KGaA
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31 May 2012
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Assay kit for the determination by flow cytometry or fluorescence microscopy of caspase-3-like activity in intact cells undergoing apoptosis. Can be used to evaluate the ability of a test substance or drug candidate to activate or inhibit caspase-3. The assay is based on the reaction of the enzyme with the cell-permeable PhiPhiLux® G2D2 substrate. Each substrate molecule contains two fluorophores and the cleaved substrate fluoresces red.
| Product information | |||
|---|---|---|---|
| Format | Flow cytometry or fluorescence microscopy | ||
| Form | 30 Tests | ||
| Detection method | Fluorescence | ||
| Avoid freeze/thaw | Yes | ||
| Assay time | 1.5-2 h | ||
| Sample type | Intact cells | ||
| Kit contains | Flow Cytometry Buffer, PhiPhiLux® G2D2 Substrate, and a user protocol. | ||
| Excitation max. | ~552 nm | ||
| Emission max. | ~580 nm | ||
| Declaration | Sold under license of U.S. Patents 5,605,809 and 5,714,342. Not available for sale in Japan. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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