For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
31 May 2012
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A convenient and sensitive method for the detection of activated caspases in living cells. The fluorescent marker is VAD-FMK conjugated to sulforhodamine. This cell-permeable, non-toxic inhibitor binds irreversibly to activated caspases in apoptotic cells. The fluorescence intensity can be measured by fluorescence microscopy, fluorescence microplate reader or flow cytometry.
| Product information | |||
|---|---|---|---|
| Format | Flow cytometry or fluorescence microscopy | ||
| Form | 100 Tests | ||
| Detection method | Fluorescence | ||
| Species reactivity | a broad range of species | ||
| Avoid freeze/thaw | Yes | ||
| Assay time | 1.5 h | ||
| Sample type | Intact cells | ||
| Kit contains | Labeled Caspase Inhibitor (Red-VAD-FMK), Wash Buffer, Caspase Inhibitor (Z-VAD-FMK), and a user protocol | ||
| Excitation max. | ~540 nm | ||
| Emission max. | ~570 nm | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 23-26-36 Do not breathe fumes. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. |
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| R Phrase |
R: 36/37/38 Irritating to eyes, respiratory system and skin. |
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