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Merck KGaA
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Phone: +49 6151 72-0
Fax: +49 6151 72 2000
31 May 2012
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Detects and quantifies the level of CREB (cAMP-Response Element-Binding protein) independent of its phosphorylation state in human and mouse cells. CREB is a member of the leucine zipper family of DNA binding proteins. It induces the transcription of target genes in response to several stimuli such as peptide hormones and growth factors.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, mouse | ||
| Specificity | Human and mouse CREB with slight cross-reactivity with CREM | ||
| Sensitivity | < 0.025 ng/ml | ||
| Assay range | 0.156-10 ng/ml | ||
| Assay time | 4 h | ||
| Sample type | Cells | ||
| Positive control | HeLa, Jurkat, HT1080, and NIH3T3 cells | ||
| Kit contains | CREB Standard, Standard Diluent Buffer, CREB Antibody-Coated 96-Well Plate, Rabbit Anti-CREB (Total) Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 26-36 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. |
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| R Phrase |
R: 25-36/37/38 Toxic if swallowed. Irritating to eyes, respiratory system and skin. |
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| Data | |||
|---|---|---|---|
 The sensitivity of this ELISA was compared to immunoblotting using known quantities of CREB. The data presented in Figure 1 show that the sensitivity of the ELISA is ~4x greater than that of immunoblotting. The bands shown in the immunoblot data were developed using an anti-CREB antibody and, chemiluminescent detection. |
