D-Tube™ Dialyzers

D-Tube™ Dialyzers are easy-to-handle dialyzers in a capped centrifuge tube format with dialysis membrane windows for buffer exchange and removal of solutes like urea or detergents while providing > 97% sample volume recovery from solution. The disposable, single-use tubes require no syringes, centrifuge, or laborious steps to manipulate small sample volumes. The sample is added and removed using a standard laboratory pipet. D-Tube™ Dialyzers are available with molecular weight cut-offs from 3.5 to 14 kDa and are designed with six volume capacities: mini (10–250 μl), midi (50–800 μl), maxi (100– 3000 μl), and mega (10, 15, and 20 μl). The membrane is ultra-clean, EDTA-treated regenerated cellulose, sulfur- and heavy metal-free. Each kit contains 10 D-Tube™ Dialyzers (Mega kits available in 10 and 50 pk) and one floating rack that can hold up devices in an exchange buffer.

• Easy-to-handle dialyzers for buffer exchange and removal of urea and detergents for samples ranging from 10 ul to 20 ml
• One-step dialysis procedure that does not require syringes or any special equipment
• Efficient sample recovery
• Protease-, RNase-, and DNase-free
• Ideal for electroelution of proteins, protein-DNA complexes, oligonucleotides, DNA, and RNA from polyacrylamide and agarose gels
• Procedure compatible with variety of downstream applications including MALDI-MS, functional assays, and HPLC
• D-Tube96™ allows for simultaneous electroelution from multiple samples
• No sample loss or contamination, just high yields.

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Electroelution

Following separation of biomolecules by agarose or polyacrylamide gel electrophoresis, electroelution offers a means to extract the sample of interest by applying an electric current to the excised gel band. The combination of D-Tube™ Dialyzers and the D-Tube Electroelution Accessory Kit provides a unique tool for extraction of any protein, protein-protein, or protein- DNA complexes from non-denaturing and denaturing (SDS) polyacrylamide gels, and for extraction of oligonucleotides, RNA, and DNA from both polyacrylamide and agarose gels. The D-Tube Electroelution Accessory Kit provides three D-Tube supporting trays that fit into most horizontal electrophoresis units and optimized reagents for protein and nucleic acid precipitation following electroelution.

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D-Tube96

The D-Tube96™ Dialyzer allows convenient and simultaneous dialysis of 96 samples in a high throughput manner. The device takes advantage of the features of the D-Tube™ Dialyzer Mini in a 96-tube configuration. D-Tube™ Dialyzers are easy to handle dialyzers with dual membranes and large surface area. The membrane is ultra clean, EDTA-treated regenerated cellulose, sulfur- and heavy metal- free, and available in molecular weight cutoffs of 6-8 kDa or 12-14 kDa. The D-Tube™ Dialyzers Mini have a capacity of 10-250 µl and are ideal for small samples. The samples are added and removed using a standard laboratory pipet, multichannel pipet, or by robotic dispensing. D-Tube96™ Dialyzers are suitable for dialysis of proteins, oligonucleotides, RNA, or DNA in a high-throughput format. Each kit contains a floating rack with 96 D-Tube™ Dialyzers Mini, 96 caps, and an adhesive aluminum plate sealer. If fewer than 96 tubes are required, excess tubes can be removed from the modular device and saved for use at a later time. D-Tube96™ Dialyzers are ideally suited for buffer exchange of protein samples into a physiologically relevant buffer after screening for optimal refolding conditions with the iFOLD™ Protein Refolding Systems.

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The Floating Rack is designed to hold D-Tube™ Dialyzers

The Floating Rack is designed to hold D-Tube™ Dialyzers in an exchange buffer. Each Floating Rack, Mini can hold up to nine D-Tube™ Dialyzer, Mini devices. Each Floating Rack, Midi can hold up to four D-Tube™ Dialyzer, Midi units. Each Floating Rack, Maxi can hold up to seven D-Tube™ Dialyzer, Maxi devices. Each Floating Rack, Mega can hold one D-Tube™ Dialyzer, Mega unit. Each package contains 10 Floating Racks.

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