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01 June 2012
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Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
| Product information | |||
|---|---|---|---|
| Form | White lyophilized solid | ||
| Formulation | Lyophilized from 10 mM Tris-HCl, pH 7.5. | ||
| Molar mass | 27000 | ||
| Specific activity | ≥20,000 units/mg protein | ||
| Unit definition | One unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL™ Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5. | ||
| Purity | ≥90% by SDS-PAGE | ||
| Solubility | H2O | ||
| EC number | 3.4.24.33 | ||
| CAS number | 9001-92-7 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Ambient Temperature Only
Harmful |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 22-26-36 Do not breathe dust. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. |
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| R Phrase |
R: 20-36/37/38-42/43 Harmful by inhalation. Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation and skin contact. |
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