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01 June 2012
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Detects and quantifies the level of ERK 1/2 proteins independent of their phosphorylation state. The ERKs (Extracellular Signal Regulated Kinases) are members of Mitogen Activated Protein Kinases (MAPK) family of proteins that are activated by a variety of mitogenic stimuli as well as differentiation signals. The ERK 1/2 signaling cascade has been shown to be a critical regulator of cell differentiation, cell physiology and neuronal function. Although, this kit is designed for use with human cell lines, platelets, and lymphocytes, it cross-reacts with mouse and rat cells.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, mouse, rat | ||
| Sensitivity | < 16 pg/ml | ||
| Assay range | 31.2-2000 pg/ml | ||
| Assay time | 4 h | ||
| Sample type | Cells | ||
| Kit contains | Coated 96-Well Plate, ERK 1/2 Standard, Diluents, Detector Antibody, Secondary Antibody, Wash Buffer, Sample Treatment Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Data | |||
|---|---|---|---|
 The sensitivity of this ELISA was compared to immunoblotting using known quantities of ERK1/2. The data presented in Figure 4 show that the sensitivity of the ELISA is ~4x greater than that of immunoblotting. The bands shown in the immunoblot data were developed using an anti ERK1/2 antibody and chemiluminescent detection. |
