70850  FastPlax™ Titer Kit

Rapid, reliable titering of baculovirus stocks

The FastPlax™ Titer Kit is designed for determination of baculovirus titers in just 24 to 48 hours instead of three to four days as in other methods. Classical determination of baculovirus titer has relied on the appearance of plaques, but the FastPlax Kit takes advantage of the appearance of detectable levels of AcNPV gp64 glycoprotein on the cell surface as early as 8 to 24 hours postinfection. Detection is based on a high affinity monoclonal antibody against gp64, which is added directly to fixed cells. Antibody binding is detected with Goat Anti-Mouse IgG β-Galactosidase Conjugate followed by enhanced color development with X-Gal/NBT substrates. Plaques are clearly distinguished by their dark blue color. The kit contains sufficient reagents to perform five 6-well plate assays.

The FastPlax Antibody is used to detect the appearance of AcNPV gp64 glycoprotein on the surface of infectted cells as early as 8-24 hours post-infection. This forms the basis of the FastPlax Titer Kit, which allows determination of baculovirus titers in just 25-48 hours.

The FastPlax Antibody can also be used to detect gp64 on Western blots.