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Merck KGaA
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01 June 2012
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A spectrophotometric assay kit used for the measurement of reduced to oxidized glutathione ratio (GSH/GSSG) in biological samples.
| Product information | |||
|---|---|---|---|
| Format | Cuvette | ||
| Form | 200 Tests | ||
| Detection method | Colorimetric | ||
| Protect from Light | Yes | ||
| Sensitivity | 9 nM GSSG | ||
| Assay time | 1.5 h | ||
| Sample type | cell lysates, tissue extracts, blood | ||
| Kit contains | Chromogen DTNB, Glutathione Reductase Enzyme, NADPH, Scavenger, Assay Buffer, GSSG Buffer, Standard, and a user protocol. Each kit can be used to perform 100 tests representing the ratio of total Glutathione to the oxidized form or 200 tests of either. | ||
| Features and benefits |
• Highly Sensitive and Specific:
Lower limit of detection in sample mixture is 9 nM GSSG. Over 13 different compounds were tested to assess any interference and exhibited no interference.
• Easy to Use: A simplified colorimetric assay. the GSH/GSSG assay uses the thiol-scavenging reagent that rapidly scavenges GSH, but does not interfere with glutathione reductase assay. Each kit can be used for up to 100 tests for determining total glutathione/GSSG ratios or 200 tests for either of the two. Assay time: About 1.5 h • Versatile: Can be used with whole blood, cell lysates, and tissue extracts. |
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| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 A four point calibration curve A<sub>412</sub>/min VS. µ GSH used to determine the concentration of GSSG.  Stability of GSSG, from a rested subject, in the intact red blood cell at 4°C. (GSH-159).  Stability of GSSG in the red blood cell lysate at 4°C. (GSH-161) |
