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CBA064  IκBα ELISA Kit

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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000

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31 May 2012

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Detects and quantifies the level of IκBα independent of its phosphorylation state. IκBα belongs to IκB protein family and is known to modulate NF-kB (nuclear factor-κB) nuclear translocation. It plays an important role in immune and inflammatory responses, cell division and apoptosis.
Product information
Format 96-well plate
Form 96 Tests
Detection method Colorimetric
Species reactivity human, not mouse, not rat
Sensitivity < 30 pg/ml
Assay range 62.5-4000 pg/ml
Assay time 4 h
Kit contains IκBα Standard, Standard Diluent Buffer, IκBα Antibody-Coated 96-Well Plate, Rabbit Anti-IκBα Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers and a user protocol.
Store and ship information
Storage +2°C to +8°C
Ship Blue Ice Only
Multiple Toxicity Values, refer to MSDS
Data

Known quantities of IκBα protein were measured as outlined in the Detailed Protocol and by immunoblotting using an anti-IκBα antibody and chemiluminescent detection. The ELISA is approximately 2X more sensitive than immunoblotting.

© Merck KGaA, Darmstadt, Germany, 2012


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