For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
31 May 2012
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The InnoCyte™ α2, α3, α5 Integrin Detection Kit is designed for the determination of relative levels of α2, α3, and α5 integrins on the surface of human cells (integrin profiling). The kit provides a rapid method for assessing integrin cell adhesion receptors and can be used in place of flow cytometry or immunoprecipitation. The kit is supplied with a black 96-well strip plate pre-coated with anti-mouse IgG. Antibodies specific for α2, α3, and α5 integrins are captured in the wells of the plate and cells are subsequently incubated in the wells containing the integrin antibodies. Following a brief wash step the cells are labeled with the green fluorescent dye, calcein-AM. Relative cell attachment is assessed using a fluorescence plate reader.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Fluorometric | ||
| Assay time | 3 h | ||
| Sample type | Cells | ||
| Kit contains | Goat Anti-Mouse IgG-Coated 96-Well Plate, Anti-α2 Integrin, Anti-α3 Integrin, Anti-α5 Integrin, Assay Diluent, Calcein-AM Solution, D-PBS, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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