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31 May 2012
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The InnoCyte™ Cell Adhesion Assay, Galectin-1/Galectin-3 is designed for the determination of the relative attachment of adherent cell lines to galectin-1 and galectin-3, for evaluation of cell adhesion receptors, and for assessment of inhibitory or stimulatory substances for cell attachment. The kit is supplied with a 96-well strip plate coated with galectin-1 and galectin-3. Cells are seeded in the coated wells and incubated at +37°C. Following incubation, the wells are washed briefly and attached cells are labeled the green fluorescent dye, calcein-AM. BSA-coated wells serve as a negative control and poly-L-lysine-coated wells serve as a positive control for general attachment. Relative cell attachment is assessed using a fluorescence plate reader.
• 96-well tissue culture plate coated with Galectin-1/Galectin-3
• Assay is quick-can be performed in 2.5 h
• Adherent cells are labeled with calcein-AM
• Relative cell attachment is assessed using a fluorescence plate reader
• 96-well tissue culture plate coated with Galectin-1/Galectin-3
• Assay is quick-can be performed in 2.5 h
• Adherent cells are labeled with calcein-AM
• Relative cell attachment is assessed using a fluorescence plate reader
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 72 Tests (36 Gal-1 and 36 Gal-3) | ||
| Detection method | Fluorometric | ||
| Protect from Light | Yes | ||
| Avoid freeze/thaw | Yes | ||
| Assay time | 2.5 h | ||
| Sample type | Adherent cultured cells | ||
| Kit contains | Coated 96-Well Plates, Fluorescent Dye, D-PBS, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
||
| Data | |||
|---|---|---|---|
 ~40,000 cells were added to wells coated with galectin-1, galectin-3, and BSA and incubated for 1.5 h at 37°C in the presence of 6% CO<sub>2</sub>. Cells were washed gently with D-PBS and labeled with Calcein-AM for 1 h at 37°C in the presence of 6% CO<sub>2</sub>. Fluorescence was measured as indicated in the Detailed Protocol. HT-1080 cells displayed appreciable binding to poly-L-lysine, which served as a positive control (data not shown). |
