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Merck KGaA
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31 May 2012
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A sensitive fluorogenic assay for the measurement of cathepsin B activity (Excitation max: ~360-380 nm; Emission max: ~430-460 nm). Cathepsin B is a cysteine proteinase which has been associated with increased invasivessness and development of the malignant cell phenotype. Cathepsin B has also been implicated in inflammatory airway disease.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Fluorometric | ||
| Protect from Light | Yes | ||
| Avoid freeze/thaw | Yes | ||
| Sensitivity | 0.63 ng/ml | ||
| Assay range | 0.09 - 100 ng/ml | ||
| Assay time | 30 min | ||
| Sample type | Cells and tissues | ||
| Positive control | Cathepsin B | ||
| Kit contains | Cathepsin B Enzyme, Calibration Standard, Cathepsin B Substrate, Assay Buffer, Cathepsin B Inhibitor, Reduction Reagent, Cell Lysis Buffer, Microtiter Plate, Plate Sealer, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | Multiple storage requirements | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Data | |||
|---|---|---|---|
 Calibration curve generated by various dilutions of the free AMC standard included in the kit (above). |
