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CBA002  InnoZyme™ Cathepsin D Immunocapture Activity Assay Kit, Fluorogenic

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31 May 2012

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A highly selective fluorometric assay for the quantitative in vitro detection of human cathepsin D activity and for screening cathepsin D inhibitors. This assay uses a monoclonal antibody to capture the cathepsin D. Activity is detected with an internally quenched fluorescent substrate (Excitation max.:~328 nm; Emission max: ~393 nm).
Substitutes
QIA29 Cathepsin D, Rapid Format ELISA Kit
Product information
Format 96-well plate
Form 96 Tests
Detection method Fluorogenic
Protect from Light Yes
Avoid freeze/thaw Yes
Sensitivity 0.2 ng/ml
Assay range 3.13-50 ng/ml
Assay time 1.5 h
Sample type Biological fluids and culture supernatant
Positive control Cathepsin D
Kit contains Coated 96-Well Plate, Substrate, Cathepsin D Standard, Assay Buffer, Sample Buffer, Plate Wash Buffer, Plate Sealer, and a user protocol.
Store and ship information
Storage -20°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS
Data

Increasing concentrations of Cathepsin D Standard were assayed, as outlined in the Detailed Protocol. The results represent a typical standard curve.

© Merck KGaA, Darmstadt, Germany, 2012


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