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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
31 May 2012
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A highly sensitive and selective fluorogenic assay for the detection of Cathepsin L from human and mouse samples. Cathepsin L is detected with the fluorogenic substrate Z-Phe-Arg-AMC (Exc. max: 360 nm; Emi. max: 460 nm). Interference from Cathepsin B is eliminated by the incorporation of CA 074, a specific cathepsin B inhibitor. This kit is also useful for screening cathepsin L inhibitors.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Fluorometric | ||
| Protect from Light | Yes | ||
| Avoid freeze/thaw | Yes | ||
| Sensitivity | 1.2 ng/ml | ||
| Assay range | 1.56-100 ng/ml | ||
| Assay time | 1.5 - 2 h | ||
| Sample type | Cell lysates, tissues, or purified cathepsin L | ||
| Positive control | Cathepsin L | ||
| Kit contains | Cathepsin L Substrate, AMC Calibration Standard, Cathepsin L Control, Cathepsin L Activator, CA 074, Cathepsin L Inhibitor, Assay Buffer, Sample Buffer, Diluent, Microwell Plate, Plate Sealer, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
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Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. |
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| Storage | Multiple storage requirements | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Data | |||
|---|---|---|---|
 Calibration curve generated using the AMC Calibration Standard included in the InnoZyme™ Cathepsin L Activity Kit. The assay was carried out as specified in the Cathepsin L Inhibitor Screening protocol. |
