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31 May 2012
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Dipeptidyl peptidase IV (DPP IV) is a serine exopeptidase. The natural substrate of this enzyme include chemokines, cytokines, endomorphines, hormones and the pancreatic polypeptide of the PACAP/glucagons peptide family. Of particular interest is the ability of DPP IV to regulate incretin hormones. Since these hormones are deactivated by DPP IV, a multivalent method of treatment for type 2 diabetes and, more generally, in glucose metabolism disorders, is opened by the inhibition of this protease.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Fluorescence | ||
| Species reactivity | human | ||
| Specificity | Human DPP IV | ||
| Assay range | 0.31-20 ng/ml | ||
| Kit contains | Anti-DPP IV Antibody 96-well Coated Plate, DPP IV Control, DPP IV Substrate, Assay Buffer, 10X Sample Buffer, Plate Sealer, and a user protocol | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Irritant |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 26-37/39-56 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable gloves and eye/face protection. Dispose of this material and its container at hazardous or special waste collection point. |
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| R Phrase |
R: 36/37/38 Irritating to eyes, respiratory system and skin. |
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| Data | |||
|---|---|---|---|
  The activities of recombinant human DPP II, FAP, and DPP I were assessed as outlined in the Detailed Protocol above.  Human recombinant DPP IV activity was measured as outlined in the Detailed Protocol above using a 1-h incubation at 37°C. The data is presented as Relative Fluorescence Units.  Cells (cell types are denoted in the figure) were cultured in DMEM with 10% FCS and harvested at 80-90% confluency. Total cell lysates were prepared using CytoBuster™ Protein Extraction Reagent (Cat. No. 71009) according to the manufacturer's recommended protocol. Human spleen extract was prepared by homogenizing tissue with 170 mM NaCl, 2 mM EDTA. Human plasma (pooled samples) was obtained from commercial sources. The concentration of active DPP IV was determined by extrapolating the RFU values to the standard curve prepared with human recombinant DPP IV. Protein concentration in cell lysates and tissue extract was determined using a BCA protein assay. Activity was assessed as outlined in the Detailed Protocol above. Fluorescence intensity was measured at an excitation wavelength of 360 nm and an emission wavelength of 460 nm and expressed per mg total protein. |
