CBA003
InnoZyme™ Gelatinase (MMP-2/MMP-9) Activity Assay kit, Fluorogenic
MMP-2/MMP-9 Activity Assay Kit, Fluorogenic
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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Teléfono: +49 6151 72-0
Fax: +49 6151 72 2000
31 mayo 2012
cargando
A sensitive fluorogenic assay (Exc. max: ~325 nm; Emi. max.: ~393 nm) for the measurement of gelatinases (MMP-2 and MMP-9). Also useful for the screening of gelatinase inhibitors.
| Información sobre producto | |||
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| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Fluorogenic | ||
| Protect from Light | Si | ||
| Avoid freeze/thaw | Si | ||
| Sensitivity | 20 ng/ml | ||
| Assay time | 2 - 6 h | ||
| Sample type | Cell lysate, culture supernatant, tissue extract, plasma, serum, purified enzyme | ||
| Positive control | MMP-9 (Cat. No. PF140) | ||
| Kit contains | Gelatinase Substrate, pro-MMP-2 Positive Control, Inhibitor, APMA, Assay Buffer, 96-Well Plate, Plate Sealer, and a user protocol | ||
| Almacenar y enviar información | |||
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Advertencia de materiales peligrosos: Debido a la naturaleza de los materiales peligrosos de este envío, podrían aplicarse gastos de envío añadidos a su pedido. Ciertos tamaños pueden estar exentos de los gastos de envío añadidos a los materiales peligrosos. Consulte a su oficina local de ventas si desea más información relativa a esos gastos. |
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| Categoría de alamacenamiento | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Datos | |||
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 Human Pro-MMP-2 (supplied) was activated as outlined in the Reagent Preparation section above and assayed for activity as outlined in the Detailed Protocol, incubating for a total of 3 h.  Activated MMP-9 (Cat. No. PF140) was assayed without APMA at 37°C for 3 h according to the Detailed Protocol outlined above.  Serum from 5 different donors was diluted 1:5 in Activation Buffer and HT1080 culture supernatant was diluted 1:3 with Activation Buffer. All samples were incubated at 37°C for 3 h according to the Detailed Protocol outlined above. |
