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31 May 2012
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A sensitive and specific fluorogenic assay for Granzyme B based on the QuickZyme™ technology platform where modified prourokinase serves as a substrate for Granzyme B. Cleavage by Granzyme B activates the urokinase and activity is determined with a urokinase substrate. Specificity is increased through the immunocapture of Granzyme B on the microtiter plate. Granzyme B is a caspase-like serine protease with proposed anti-apoptotic, antiviral, and antitumor functions.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Fluorometric | ||
| Protect from Light | Yes | ||
| Avoid freeze/thaw | Yes | ||
| Sensitivity | 0.01 ng/ml (5 h incubation) and 0.3 pg/ml (24 h incubation) | ||
| Assay range | 0.0625- 2 ng/ml (5 h incubation) and 3.125 - 50 pg/ml (24 h incubation) | ||
| Assay time | 7 - 30 h | ||
| Sample type | Cell lysates, tissue extracts, and body fluids | ||
| Positive control | Granzyme B | ||
| Kit contains | Coated 96-Well plate, Modified Prourokinase (UKGran), Substrate, Granzyme B Standard, Assay Buffer, Wash Buffer, Plate Sealers, and a user protocol. | ||
| Excitation max. | ~360-380 nm | ||
| Emission max. | ~460-480 nm | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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