CBA042
InnoZyme™ TACE Activity Kit
α-Secretase Activity Kit, TNF-α Converting Enzyme Activity Kit, ADAM17 Activity Kit
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
31 May 2012
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The InnoZyme™ TACE Activity Kit is a specific and sensitive assay designed to measure human TACE activity in cell lysates and biological samples and for screening enzyme inhibitors. An Anti-Human TACE-Coated 96-Well Plate is pre-coated with a monoclonal antibody specific for human TACE that captures the enzyme. Unbound material is discarded, the plate is washed, and the activity of captured TACE is measured using an internally quenched fluorescent substrate, MCA-KPLGL-Dpa-AR-NH2. Cleavage of the scissile amide bond, G-L, releases the fluorophore from the quenching molecule, Dpa, resulting in an increase in fluorescence. Fluorescence of the cleaved product, MCA-KPLG, is measured at an excitation wavelength of ~324 nm and emission wavelength of ~400 nm. The level of fluorescence is directly related to the enzyme activity.
• Highly specific: based on immunocapture of human TACE
• Highly sensitive and quantitative: assay range 5-100 ng/ml
• Convenient: 96-well format and non-radioactive detection
• Versatile: appropriate for measuring active TACE and high-throughput screening of TACE inhibitors
• Highly specific: based on immunocapture of human TACE
• Highly sensitive and quantitative: assay range 5-100 ng/ml
• Convenient: 96-well format and non-radioactive detection
• Versatile: appropriate for measuring active TACE and high-throughput screening of TACE inhibitors
| Product information | ||||
|---|---|---|---|---|
| Format | 96-well plate | |||
| Form | 96 Tests | |||
| Detection method | Fluorometric | |||
| Protect from Light | Yes | |||
| Avoid freeze/thaw | Yes | |||
| Components |
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| Assay range | 5-100 ng/ml as measured with purified recombinant human TACE | |||
| Sample type | Cells | |||
| Positive control | Human recombinant TACE | |||
| Kit contains | Anti-Human TACE-Coated 96-Well Plate, Control, Substrate, Sample Buffer, Assay Buffer, Wash Buffer, Plate Sealer, and a user protocol. | |||
| Declaration | Not available for sale in Germany. | |||
| Store and ship information | |||
|---|---|---|---|
| Storage | Multiple storage requirements | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Data | |||
|---|---|---|---|
 The activity of increasing concentrations human recombinant TACE (Cat. No. PF133) was measured according to the Detailed Protocol above.  Human colorectal adenocarcinoma cells, DLD1 and HT-29 and human glioblastoma cells, T98G, were cultured in DMEM medium supplemented with 10% FCS and harvested at 80-90% confluency. Total cell lysates were prepared using CytoBuster™ Protein Extraction Reagent (Cat. No. 71009) and the manufacturer's recommended protocol. TACE activity was measured according to the Detailed Protocol above. RFU is reported per mg protein. |
