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444168  InSolution™ MANT-GppNHp

mant-GMPPNP, 2′/3′-O-(N-Methylanthraniloyl)-guanosine-5′-[(β,γ)-imido]triphosphate, Triethylammonium Salt, 2′/3′-O-(N-Methylanthraniloyl)-β,γ-imidoguanosine-5′-triphosphate, Triethylammonium Salt

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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000

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01 June 2012

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A more lipophilic, fluorescent derivative of the GTP-hydrolysis-resistant GTP analog, GppNHp. Useful for investigating the interactions of low molecular weight GTP-binding proteins with their specific effector proteins. Acts as a potent, competitive inhibitor of adenylyl cyclase (AC; Ki = 161 nM and 155 nM in forskolin/Mn2+-stimulated AC in S49 cyc- membranes and insect cell membranes, respectively). Shown to bind to Go-proteins with high affinity, Gsα-Giα-proteins with low affinity, and ineffective at activating Gα.
Product information
Form Liquid
Formulation A 5 mM solution in H2O.
Primary Target AC
Primary Target Ki 161 nM and 155 nM in forskolin/Mn2+-stimulated AC in S49 cyc- membranes and insect cell membranes, respectively
Molar mass 958.9
Protect from Light Yes
Purity ≥90% by HPLC
Excitation max. 280 or 355
Emission max. 440
Chemical formula C18H21N7O14P3 • C18H48N3
Store and ship information
Storage ≤ -70°C
Ship Dry Ice Only
Standard Handling
InSolution™ MANT-GppNHp, molecular structure

© Merck KGaA, Darmstadt, Germany, 2012


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