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01 junio 2012
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The K-LISA™ Checkpoint Activity Kit is a rapid, sensitive, ELISA-based activity assay suitable for measuring the kinase activity of purified or partially purified Chk1 and Chk2 preparations, in vitro Chk1 and Chk2 inhibitor screening, and for assessing the regulation of Chk1 and Chk2 in cell signaling. The assay utilizes a biotinylated peptide substrate (KKKVSRSGLYRSPSMPENLNRPR) that is phosphorylated on the third serine by Chk1 and Chk2. Biotinylated Chk substrate and sample containing Chk1 or Chk2 are incubated in the presence of ATP in wells of the streptavidin-coated 96-well plate, which allows for phosphorylation and substrate capture in a single step.
• Highly versatile: can detect kinase activity in purified or partially purified Chk1 and Chk2 preparations.
• Highly sensitive: can detect kinase activity at picogram (Chk1) and nanogram levels (Chk2).
• Highly convenient: 96-well format, non-radioactive detection.
• Highly useful: enables high throughput screening of kinase inhibitors.
• Highly versatile: can detect kinase activity in purified or partially purified Chk1 and Chk2 preparations.
• Highly sensitive: can detect kinase activity at picogram (Chk1) and nanogram levels (Chk2).
• Highly convenient: 96-well format, non-radioactive detection.
• Highly useful: enables high throughput screening of kinase inhibitors.
| Producto alternativo | |
|---|---|
| CBA020 | K-LISA™ Checkpoint Activity Kit |
| Productos alternativos para K-LISA™ Checkpoint Activity Kit | |
| CBA020 | K-LISA™ Checkpoint Activity Kit |
| Accesorios para K-LISA™ Checkpoint Activity Kit |
| Información sobre producto | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Avoid freeze/thaw | Si | ||
| Sensitivity | 25 pg (0.018 mU) purified Chk1 | ||
| Assay range | 25-725 µU (Chk1) and 260 µU - 26 mU (Chk2) | ||
| Assay time | 3 h | ||
| Sample type | Cell lysates, tissue extracts, purified enzymes | ||
| Kit contains | Biotinylated Chk1 Substrate, Phosphoserine Detector Antibody, Streptavidin Coated 96-Well Plate Strips, HRP-Conjugated Secondary Antibody, Serine/Threonine Kinase Assay Buffer, ATP/MgCl2, Chk1 Inhibitor, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. Purified Chk1 is available separately (Cat. No. 220479). | ||
| Almacenar y enviar información | |||
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Advertencia de materiales peligrosos: Debido a la naturaleza de los materiales peligrosos de este envío, podrían aplicarse gastos de envío añadidos a su pedido. Ciertos tamaños pueden estar exentos de los gastos de envío añadidos a los materiales peligrosos. Consulte a su oficina local de ventas si desea más información relativa a esos gastos. |
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| Categoría de alamacenamiento | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Datos | |||
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 The activity of His<b>•</b>Tag<sup>®</sup>, Human, Recombinant Chk1 (Cat. No. 220479) (17-2200 pg) was determined using protocol A as indicated in the Detailed Protocol section. Assay range: 34 pg-1100 pg (740 Units/mg).  The activity of Human, Recombinant Chk2 (0.06-60 ng) was determined using protocol A in the Detailed Protocol section. Assay range: 200 pg-20 ng (1283 Units/mg). |
