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Merck KGaA
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01 June 2012
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An ELISA-based activity assay for measuring the kinase activity of purified or mTOR immunoprecipitated from cell lysates. Useful for in vitro mTOR inhibitor screening and for assessing the regulation of mTOR cell signaling. This assay utilizes a p70S6K GST fusion protein as a specific mTOR substrate.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Avoid freeze/thaw | Yes | ||
| Sample type | Purified or partially purified enzyme | ||
| Kit contains | Glutathione-Coated 96-Well Plate, mTOR Substrate, Anti-p70S6K-T389, mTOR Standard, 2X Kinase Assay Buffer, ATP Solution, 100 mM DTT, Wortmannin 50X, Kinase Stop Solution, 10X Plate Wash Concentrate, Antibody Diluent, HRP-Antibody Conjugate, TMB Substrate, ELISA Stop Solution, Plate Sealers and a user protocol. | ||
| Declaration | Sold under license of U.S. Patent 7,135,298. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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