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Merck KGaA
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64293 Darmstadt
Germany
Phone: +49 6151 72-0
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01 June 2012
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The K-LISA™ PTK Screening Kit, an ELISA-based protein tyrosine kinase (PTK) activity assay, is a rapid, non-radioactive method to screen for PTK activity of purified enzyme samples as well as in cell lysates or subcellular fractions and tissue extracts. This colorimetric assay can detect activity at picomolar concentrations with purified PTKs and in as little as 5 µg total protein from cell lysates and 20 ng total protein from tissue extracts. The kit uses three different random polypeptide substrates (EAY, E4Y, or EY) that are composed of different molar ratios of glutamic acid (E), alanine (A), and tyrosine (Y), (1:1:1, 4:1, and 1:1, respectively) and are immobilized on two 96-well plates. To study general PTK activity, substrate selectivity, or changes in PTK activity associated with inhibitor or mutational effects by either comparative or ratiometric analysis.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 216 Tests, 72 Controls | ||
| Detection method | Colorimetric | ||
| Protect from Moisture | Yes | ||
| Assay time | 3 h | ||
| Sample type | Cell lysates, tissue extracts, purified enzyme | ||
| Kit contains | Two 96-well reaction plates, reaction buffer, protease inhibitor cocktail, DTT, activated sodium orthovanadate, ATP, stop solution, Peroxidase conjugated Anti-phosphotyrosine, TMB, PBST tablets, positive control, plate sealers, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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