71975 KOD Xtreme™ Hot Start DNA Polymerase

KOD system optimized for difficult targets

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21 Mai 2013

wird geladen

Bestellnummer St./Pkg. Menge Preis

71975-3

Bestellnummer	St./Pkg.	Menge	Preis
71975-3	200 u	Aktualisieren	wird geladen
Preisänderungen vorbehalten. Bitte geben Sie für mindestens eine Produktnummer eine Menge an. Diese Produkte können momentan nicht bestellt werden.

200 u

Aktualisieren

wird geladen

Preisänderungen vorbehalten.

The KOD Xtreme™ Hot Start DNA Polymerase* kit is an optimized PCR system for the amplification of long or GC-rich DNA templates. The unique formulation enables you to amplify directly from minimally processed samples; allowing highly efficient crude sample PCR target amplification. The system includes an ultra high fidelity KOD DNA polymerase complexed with two monoclonal antibodies to permit hot start thermocycling, along with specially formulated 2X buffer. KOD Xtreme Hot Start DNA Polymerase quickly and accurately amplifies genomic and phage/plasmid DNA targets up to 24 and 40 kbp, respectively. KOD Xtreme Hot Start DNA Polymerase successfully amplifies challenging DNA templates with up to 90% GC content. Each kit provides 200 U KOD Xtreme Hot Start DNA Polymerase, an optimized buffer and dNTPs sufficient for 200 amplification reactions. The polymerase produces blunt-ended DNA products suitable for cloning with the Novagen Perfectly Blunt^® and LIC Vector Kits.

*Manufactured by Toyobo and distributed by EMD. Not available from Merck in Japan.

Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.

Produktinformationen

Unit definition

One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl₂, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), and 150 µg/ml activated calf thymus DNA.

Components

•1 × 200 U KOD Xtreme™ Hot Start DNA Polymerase

•3 × 1.7 ml 2X Xtreme Buffer

•2 × 1 ml dNTPs (2 mM each)

Features and benefits

Optimized for the highest PCR success rate, even with the most difficult targets
10X higher fidelity than Taq blends
Efficiently amplifies up to 90% GC-content DNA templates
Amplifies genomic targets up to 24 kbp
Amplifies phage/Lambda targets up to 40 kbp
Eliminates mispriming and primer-dimer formation
Convenient ambient-temperature setup compatible with automation
See Figures at the bottom of this page for addtitional product information