428900
LCAT Activity Assay Kit, Fluorometric
Lecithin:Cholesterol Acyltransferase Activity Assay Kit, Fluorometric
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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Assay kit useful for the quantitative measurement of lecithin:cholesterol acyltransferase activity (LCAT) in human plasma. LCAT mediates the formation of cholesteryl esters in human plasma by transferring an acyl chain from the sn-2 position of phosphatidylcholine to cholesterol.
| Product information | |||
|---|---|---|---|
| Form | 100 Tests | ||
| Detection method | Fluorometric | ||
| Species reactivity | human | ||
| Protect from Light | Yes | ||
| Assay time | 4.5 - 8.5 h | ||
| Sample type | Plasma | ||
| Kit contains | LCAT Substrate, READ Reagent, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Irritant |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
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| R Phrase |
R: 36/38 Irritating to eyes and skin. |
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| Data | |||
|---|---|---|---|
 The non-hydrolyzed LCAT Substrate fluoresces at 470 nm. Upon hydrolysis by LCAT, a monomer is released from the LCAT Substrate that fluoresces at 390 nm. LCAT activity is assessed as a change in 470/390 emission intensity.  Normal human plasma is assayed according to the Detailed Protocol above. Over time the 470/390 ration decreases, indicating LCAT activity in the sample. |
