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70716  MagPrep® Streptavidin Beads

Magnetic isolation of biotinylated molecules

For general questions please contact our Customer Service:

Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000

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01 June 2012

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This product is being discontinued. It will be available until 9/30/2009 or while supplies last. We are offering an improved product, MagPrep® P-25 Streptavidin Beads (Cat. No. 72190) as an alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.

MagPrep® Streptavidin Beads are encapsulated super-paramagnetic polystyrol particles coated with streptavidin. The beads have a diameter of 1 µm and are provided as a stabilized aqueous suspension of 10 mg/ml. The special coating and dense layer of streptavidin on the surface guarantee maximum binding capacity with minimal nonspecific binding. The small size of the beads ensures that they remain suspended in solution during the binding process and the high magnetite content (40%) allows rapid separation from the aqueous phase using a magnet. MagPrep Streptavidin Beads are optimized for molecular applications such as hybrid capture techniques, e.g., isolation and enrichment of specific DNA sequences or mRNAs using appropriate biotinylated probes or primers. The beads are compatible with downstream applications such as sequencing and cDNA synthesis, and can also be used for isolation of biotinylated antibodies or other biotinylated targets.

Specifications
Concentration10 mg/ml (1010 beads/ml)
Storage bufferPBS, 0.1 mg/ml BSA, 0.05% Proclin 300
Iron content40%
Density1.4 g/ml
Sizeapproximately 1 µm
Binding capacity> 400 pmol free biotin/mg
> 400 pmol biotinylated oligonucleotide/mg (10-20 nt)
> 5 µg biotinylated IgG/mg

Product information
Do not freeze Yes
Store and ship information
Storage +2°C to +8°C
Ship Shipped at Ambient Temperature or with Blue Ice
Standard Handling

© Merck KGaA, Darmstadt, Germany, 2012


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