444248
MMP-13, Proenzyme, His•Tag®, Human, Recombinant, S. frugiperda
Collagenase-3
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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Full-length recombinant, human MMP-13 fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. This 452 amino acid proenzyme contains the N-terminal propeptide, the Ca2+- and Zn2+-binding catalytic domain, the hinge region, and the C-terminal hemopexin domain. Hydrolyzes collagen type II 5 to 6 times faster than collagens Type I and III. Exhibits high activity towards gelatin and degrades α1-antichymotrypsin and plasminogen activator inhibitor-2 (PAI-2).
Note: 1 mU = 1 milliunit.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl2, pH 7.5. | ||
| Molar mass | 60000 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥50 mU/mg protein | ||
| Unit definition | One unit is defined as the amount of APMA-activated enzyme that will hydrolyze 1.0 µmol MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (Cat. No. 03-32-5032) per min at 37°C, pH 7.5. | ||
| Purity | ≥90% by SDS-PAGE | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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