PF023
MMP-2, Active, Human, Recombinant, CHO Cells
Gelatinase A, Active Matrix Metalloproteinase 2
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01 June 2012
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Full-length, recombinant, human pro-MMP-2 expressed in CHO cells that is subsequently activated by APMA. APMA is removed through a desalting column. The substrate specificity for MMP-2 includes collagen (types IV, V, VII, and X), elastin, and gelatin (type I). The presence of TIMP-2 inhibitor prevents degradation of the MMP-2 C-terminal regulatory domain. TIMP-2 is also an inhibitor of proteolysis and will inhibit the activity of the enzyme. Useful for immunoblotting, substrate cleavage assay and zymography.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 50 mM HEPES, 10 mM CaCl2, 20% glycerol, 0.005% BRIJ®-35 Detergent, pH 7.5. | ||
| Preservative | None | ||
| Molar mass | 66000 | ||
| Avoid freeze/thaw | Yes | ||
| Activity | ΔΑ405/hour/µg protein: ≥ 7.0 in standard thiopeptolide hydrolysis assays. | ||
| Purity | ≥90% by SDS-PAGE; contains 9% TIMP-2 | ||
| EC number | 3.4.24.24 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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