N-a-Fmoc protected amino acids attached to NovaSyn® TGT resin
NovaSyn® TGT resins consist of NovaSyn® TG resin derivatized with Bayer's extremely acid-sensitive 4-carboxytrityl linker. The 90 µm beads have a narrow size distribution, excellent pressure stability and swelling properties, and high diffusion rates, making them ideally suited for both batch or continuous flow peptide synthesis. Cleavage can be effected by treatment with AcOH/TFE/DCM (2:2:6), 0.5% TFA in DCM, or 30% HFIP in DCM, to yield protected peptide acid fragments without any loss of sensitive side-chain protecting groups. Because enantiomerization can not occur during attachment of the first residue to TGT resin, peptides containing C-terminal Cys and His residues can be produced free from contamination by diastereomeric side-products. This support is also particularly suited to the synthesis of prolyl peptides as the bulk of the trityl handle prohibits diketopiperazine formation.