For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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Assay kit useful for the rapid quantitative measurement of nitric oxide synthase (NOS) activity. It is based on the conversion of radioactive L-arginine to L-citrulline by NOS. Radioactive citrulline is separated from unreacted arginine on a cation-exchange resin and quantitated. Note: It is necessary to check the radiopurity of 3H-arginine before proceeding to use the kit.
| Product information | |||
|---|---|---|---|
| Format | Scintillation vial | ||
| Form | 50 Tests | ||
| Detection method | Radiometric | ||
| Sensitivity | picomolar range | ||
| Assay range | 2 h | ||
| Sample type | Tissue or cell extracts | ||
| Kit contains | Homogenization Buffer, Reaction Buffer, NADPH, CaCl2, Calmodulin, Stop Solution, Rat Brain Cerebellum Extract, NG-Nitro-L-Arginine Methyl Ester (L-NAME), Equilibrated Resin, Spin Cups, Cup Holders, and a user protocol. Radioactive arginine is not provided with this kit. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +15°C to +30°C | ||
| Ship |
Ambient Temperature Only
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 26-36 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. |
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| R Phrase |
R: 22-36/37/38 Harmful if swallowed. Irritating to eyes, respiratory system and skin. |
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