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71232  pBiEx™-3 DNA

InsectDirect™ System- pBiEx™-3 DNA

For general questions please contact our Customer Service:

Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000

Contact us


01 June 2012

loading
 
Product number Qty/Pk Quantity Price
71232-3  20 μg  loading
Prices are subject to change without notice.
The pBiEx™ vectors are designed for cloning and high-level expression of proteins in both bacteria and in transiently transfected Spodoptera-derived insect cells. In E. coli, target gene transcription is regulated by the tightly controlled T7lac promoter, and a ribosome binding site (rbs) directs translation initiation. In insect cells, transcription is driven by the AcNPV-derived hr5 enhancer and IE1 immediate early promoter.

pBiEx-3 contains C-terminal S•Tag and His•Tag coding sequences. For sequencing or PCR of recombinants in pBiEx-3, refer to the table below to choose the appropriate primers.

This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
Vector5' Primer3' Primeralt 5' Primer
pIEx-1S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
pIEx-2S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
pIEx-3S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
pIEx-4IE1 Promoter PrimerAS S•Tag 18mer Primer
NA
pIEx-5IE1 Promoter PrimerAS S•Tag 18mer Primer
NA
pBiEx-1S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
T7 Promoter Primer
pBiEx-2S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
T7 Promoter Primer
pBiEx-3IE1 Promoter PrimerAS S•Tag 18mer PrimerT7 Promoter Primer

Store and ship information
Storage -20°C
Ship Shipped with Blue Ice or with Dry Ice
Standard Handling
Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

© Merck KGaA, Darmstadt, Germany, 2012


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