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01 junio 2012
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Fast, sensitive assay for the in vitro quantitation of PCNA protein. The kit has broad reactivity, and is suitable to detect PCNA in autoimmune sera samples. The assay was validated using the BrdU Cell Proliferation Assay (Cat. No. QIA58).
Proliferating cell nuclear antigen (PCNA) has no known enzymatic activity; however, it can interact with a variety of proteins and regulate their activities. These varied interactions with DNA metabolic proteins imply that PCNA is a central factor for the coordination of DNA replication, DNA repair, epigenetic inheritance, and cell-cycle control.
| Información sobre producto | |||
|---|---|---|---|
| Format | 96-well strip plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, mouse, rat | ||
| Do not freeze | Si | ||
| Unit definition | One unit (U) is defined as the amount of PCNA measured from ~ 2.6 x 103 SW620 cells. | ||
| Specificity | Human PCNA protein | ||
| Assay range | 3.125-200 ng/ml of PCNA | ||
| Assay time | 3.5 h | ||
| Sample type | Cell lysates, tissue culture media , serum, or plasma | ||
| Kit contains | 96-Well Removable Strip Plate, 2 vials of PCNA Standard, Detector Antibody, 400X Conjugate, Conjugate Diluent, Substrate, Sample Diluent, 20X Plate Wash Concentrate, Antigen Extraction Agent, Stop Solution, Plate Sealers, and a user protocol. | ||
| Almacenar y enviar información | |||
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Advertencia de materiales peligrosos: Debido a la naturaleza de los materiales peligrosos de este envío, podrían aplicarse gastos de envío añadidos a su pedido. Ciertos tamaños pueden estar exentos de los gastos de envío añadidos a los materiales peligrosos. Consulte a su oficina local de ventas si desea más información relativa a esos gastos. |
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| Categoría de alamacenamiento | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Información de seguridad | |||
|---|---|---|---|
| Frase S |
S: 26-36/37/39-45 |
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| Frase R |
R: 23/24/25-35-36/37/38-40-43 |
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| Datos | |||
|---|---|---|---|
 The mean signal of each standard run in replicates of two in four assays using two different batches of plates and two different lots of detector antibody. <b>Note:</b> 44 units/ml of the old standard (lysate) corresponds to about 200 ng/ml of the new standard. |
