69054  pET-33b(+) DNA

Unique system for production of target proteins suitable for site-specific ³²P-labeling

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The pET-33b(+) vector is derived from pET-28b(+) and carries a 15 bp sequence encoding the protein kinase A (PKA) site ArgArgAlaSerVal, located between the thrombin cleavage and Nde I sites (1). Proteins expressed in pET-33b(+) can be easily purified by metal chelation chromatography (via either N- or C-terminal His•Tag^® sequences) and efficiently labeled with ³²P- or ³³P-γ-ATP and the catalytic subunit of cAMP-dependent protein kinase from heart muscle. Labeled proteins can be used as direct probes in protein-protein interaction studies. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB147). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.

The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.

This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.