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71071  pET-41 Ek/LIC Vector Kit

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01 junio 2012

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Número de producto Cant./Env. Cantidad Precio
71071-3  20 rxn  cargando
Los precios están sujetos a cambios sin notificación.
Directional PCR cloning into the most powerful E. coli expression vectors

The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. Vector encoded sequence can be completely removed by cleaving the GST fusion protein with enterokinase. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB317). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
Información sobre producto
Components
1 µgEk/LIC Vector
10 µlEk/LIC Control Insert
25 UT4 DNA Polymerase, LIC-qualified
50 µl10X T4 DNA Polymerase Buffer
100 µl100 mM DTT
50 µl25 mM EDTA
40 µl25 mM dATP
1.5 mlNuclease-free Water
22 × 50 µlNovaBlue GigaSingles™ Competent Cells
0.2 mlBL21(DE3) Competent Cells
0.2 mlBL21(DE3)pLysS Competent Cells
5 × 2 mlSOC Medium
10 µlTest Plasmid
Vector family pET
Almacenar y enviar información
Categoría de alamacenamiento ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS
Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

© Merck KGaA, Darmstadt, Alemania, 2012


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