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70790  pET Dsb Fusion System 39b plus Competent Cells

Vectors for export and periplasmic folding of target proteins

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01 junio 2012

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Número de producto Cant./Env. Cantidad Precio
Ester producto se encuentra descontinuado o no está disponible

This product has been discontinued.





The pET Dsb Fusion Systems 39b and 40b are designed for cloning and high-level expression of peptide sequences fused with the 208-aa DsbA•Tag™ sequence [pET-39b(+)] or the 236-aa DsbC•Tag™ sequence [pET-40b(+)].

DsbA and DsbC are periplasmic enzymes that catalyze the formation and isomerization of disulfide bonds, respectively (Rietsch 1996, Sone 1997, Missiakas 1994, Zapun 195, Raina 1997). In contrast to the pET-32 Trx•Tag™ series, which is designed to increase solubility in the cytoplasm, the DsbA•Tag and DsbC•Tag vectors enable potential periplasmic localization and thus may enhance solubility and proper folding in this non-reducing environment (Collins-Racie 1995).





This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
Información sobre producto
Avoid freeze/thaw Si
Components
10 µgpET vector DNA
0.2 mlHost bacterial strains BL21, BL21(DE3), and BL21(DE3)pLysS, glycerol stocks
0.2 mlInduction control clone, glycerol stock
Novagen Vector CD containing all vector sequences and maps
0.2 mlNovaBlue, BL21(DE3), and BL21(DE3)pLysS as pretested competent cells
SOC Medium
Test Plasmid
Fusion tag His•Tag, S•Tag
Almacenar y enviar información
Categoría de alamacenamiento ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS
Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

© Merck KGaA, Darmstadt, Alemania, 2012


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