70788  pET Expression System 33b plus Competent Cells

Unique system for production of target proteins suitable for site-specific ³²P-labeling

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pET Expression Systems and pET
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

Components: pET Expression Systems
Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
• 10 µg pET vector DNA (for each indicated plasmid)
• 0.2 ml BL21 Glycerol Stock
• 0.2 ml BL21(DE3) Glycerol Stock
• 0.2 ml BL21(DE3)pLysS Glycerol Stock
• 0.2 ml Induction Control Glycerol Stock

Components: pET Expression Systems plus Competent Cells
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
• 0.2 ml NovaBlue Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 2 × 0.2 ml SOC Medium
• 10 µl Test Plasmid

These components are sufficient for up to 10 transformations in each host.

Purification and Detection Reagents
Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

pET Expression System 33b plus Competent Cells


The pET-33b(+) vector is a unique expression plasmid that encodes a 5-aa recognition sequence for the catalytic subunit of cAMP-dependent protein kinase (protein kinase A; PKA) from heart muscle (Blanar 1992, de Arruda 1995). The vector-encoded fusion sequences available on pET-33b(+) include His•Tag^®, thrombin cleavage site, PKA recognition sequence (ArgArgAlaSerVal), and T7•Tag^® peptides. Fusion proteins expressed from this plasmid can be affinity purified with either His•Bind® resins or T7•Tag Antibody Agarose, and then labeled in vitro in a simple reaction with ³²P-γ-ATP and purified PKA (available in the PKAce™ Kit). Proteins can be labeled to at least 10⁵ cpm/µg in 10 minutes, and can be used directly as sensitive and specific probes for protein:protein interactions (de Arruda 1995). A powerful method that uses such probes for rapid mapping of interacting protein domains has been described (Burgess 1998, Arthur 1998).



This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.