Información
X


70620  pETBlue™-1 Blunt Vector (linearized)

Para preguntas en general por favor contacte a nuestro Servicio de Atención al Cliente:

Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Teléfono: +49 6151 72-0
Fax: +49 6151 72 2000

Póngase en contacto con nosotros


01 junio 2012

cargando
 
Número de producto Cant./Env. Cantidad Precio
70620-3  20 rxn 

Actualización
cargando
70620-4  40 rxn 

Actualización
cargando
Los precios están sujetos a cambios sin notificación.
The pETBlue™-1 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes. Screening is independent of expression because the T7lac expression promoter is in an opposed orientation relative to the E. coli promoter that mediates blue/white screening. pETBlue-1 facilitates the expression of native unfused proteins and allows convenient subcloning of target genes already fused to existing detection and purification tags. The EcoR V cloning site is appropriately spaced downstream of an E. coli ribosome binding site. Inserts must encode an ATG start codon at their 5′ end if expression is desired. The sequence is numbered from the first base of the T7 promoter sequence.


In addition to the pETBlue™ Systems, which contain uncut plasmids, pETBlue vectors are available in formats ready for insertion of PCR products. Two types of kits are available:
AccepTor™ Vector Kits and Perfectly Blunt® Cloning Kits. The AccepTor Vector Kit enables direct insertion of DNA containing single 3′-dA overhangs, such as those amplified with non-proofreading DNA polymerases (e.g., NovaTaq™ DNA Polymerase), while the Perfectly Blunt Cloning Kits are designed for cloning inserts having any type of DNA end.


Información sobre producto
Avoid freeze/thaw Si
Components
20 rxn40 rxn
•2 × 0.5 µg4 × 0.5 µgBlunt Vector
•10 µl10 µlPositive Control Insert
•100 µl2 × 100 µlEnd Conversion Mix
Features and benefits
  • No restriction enzymes or special primers
  • Compatible with any DNA polymerase
  • Independent of 3′-dA addition or sequence context of DNA ends
  • Blue/white screening with seven different vectors, including pETBlue expression vectors
  • Simple protocol takes less than one hour* from PCR product to plating transformants

* All times listed refer to kits using NovaBlue Singles™ Competent Cells and ampicillin or chloramphenicol selection. Giga kits, which use NovaBlue GigaSingles™ Competent Cells, require one hour for outgrowth.
Almacenar y enviar información
Categoría de alamacenamiento ≤ -70°C
Ship Dry Ice Only
Standard Handling

© Merck KGaA, Darmstadt, Alemania, 2012


x

Bienvenido a Merck Millipore

 

Europa, Oriente Medio y África Asia / Pacífico Norteamérica, Centroamérica y Caribe Sudamérica

Por favor elija su país:

Norteamérica, Centroamérica y Caribe


Sudamérica


Europa, Oriente Medio y África


Asia / Pacífico


Todos los países de la A a la Z


Si no encuentra su país, por favor haga clic aquí.

 

x

Merck Chemicals Cart ()

You are entering the Millipore online shop. Both carts are maintained for you while you shop between sites. We recommend to complete your Merck order before visiting Millipore.com.

Go to Millipore Proceed to checkout


What kind of products are you looking for?

Millipore

Bio manufacturing and Life science.

Merck Chemicals

High-quality industrial and laboratory chemicals.

Mensaje importante

Actualmente no es posible hacer pedidos de productos de Bioscience conjuntamente con otros productos de Merck. Le recomendamos completar primero su pedido actual.

¿Desea continuar sin completar su pedido actual? De ser así, se eliminará de su carrito de compras.