DR1002
PhosphoDetect™ Anti-ATM (pSer¹98¹) Mouse mAb (10H11.E12)
Anti-Ataxia Telangiectasia, Phospho-Specific (Ser¹98¹)
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Merck KGaA
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01 June 2012
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Recognizes the ~370 kDa ATM protein phosphorylated on Ser1981 in camptothecin-treated HeLa cells. Sold under license of U.S. Patent 7,108,992.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG1 | ||
| Immunogen | a synthetic phosphopeptide (SLAFEEGpSQSTTISS) corresponding to amino acids 1974-1988 surrounding the Ser¹98¹ phosphorylation site of human ATM | ||
| Clone | 10H11.E12 | ||
| Form | Liquid | ||
| Formulation | In PBS. | ||
| Species reactivity | human, mouse | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | HeLa cells treated with camptothecin (Cat. No. 208925) | ||
| Declaration | Sold under license of U.S. Patent 7,108,992. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Irritant |
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| Data | |||
|---|---|---|---|
 Detection of human ATM phosphorylated at Ser<sup>1981</sup> by immunoblotting. Samples: Whole cell lysate (50 µg) from untreated HeLa cells (left lane) and camptothecin-treated (100 µM) HeLa cells (right lane). Primary antibody: PhosphoDetect™ Anti-ATM (pSer<sup>1981</sup>) Mouse mAb (10H11.E12) (Cat. No. DR1002) (0.5 µg/ml). Detection: chemiluminescence.  Detection of human ATM phosphorylated at (Ser<sup>1981</sup>) by immunofluorescence. Samples: Untreated HeLa cells (left panel) and camptothecin-treated (10 µM, 2 h) HeLa cells, fixed with 100% methanol (right panel). Primary antibody: PhosphoDetect™ Anti-ATM (pSer<sup>1981</sup>) Mouse mAb (10H11.E12) (Cat. No. DR1002) (1.5 µg/ml). Detection: fluorescence. |
