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NE1036  PhosphoDetect™ Anti-Parkin (pSer378) Rabbit pAb

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25 May 2013

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Recognizes the ~52 kDa Parkin protein phosphorylated at Ser378 in HEK293 cells transfected with wild-type Parkin.
Product information
Host Rabbit
Isotype IgG
Immunogen a synthetic phosphopeptide corresponding to amino acids surrounding the Ser378 phosphorylation site of human Parkin
Form Liquid
Formulation In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Species reactivity human
Preservative None
Applications
Immunoblotting (Western Blotting)
Avoid freeze/thaw Yes
Positive control HEK293 cells transfected with wild-type Parkin
Store and ship information
Storage -20°C
Ship Blue Ice Only
Standard Handling
Data

Detection of human parkin, phosphorylated at Ser378, by immunoblotting. Sample: whole cell lysate from HEK293 cells transfected with wild-type parkin (lane 1) non-transfected HEK293 cells (lane 2). Primary antibody: PhosphoDetect™ Anti-Parkin (pSer378) Rabbit pAb (Cat. No. NE1036) (1:1000). Detection: chemiluminesence.

© Merck KGaA, Darmstadt, Germany, 2013


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