AP1063
PhosphoDetect™ Anti-PDH-E1α (pSer232) Rabbit pAb
Anti-Pyruvate Dehydogenase pSer232 Rabbit pAb
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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Telefon: +49 6151 72-0
Fax: +49 6151 72 2000
01 Juni 2012
wird geladen
Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser232 in HEK293 cells.
| Produktinformationen | |||
|---|---|---|---|
| Host | Rabbit | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Ser232 phosphorylation site of human PDH-E1α | ||
| Form | Liquid | ||
| Formulation | In PBS. | ||
| Species reactivity | human, mouse, rat | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Ja | ||
| Positive control | HEK293 cells | ||
| Lager- und Versandinformationen | |||
|---|---|---|---|
| Lagerkategorie | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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| Daten | |||
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 Detection of human PDH-E1α, phosphorylated at Ser<sup>293</sup>, Ser<sup>300</sup>, and Ser<sup>232</sup> by immunoblotting. Samples: Whole tissue extract from mouse liver (20 µg) left untreated (lane 1) or treated with dichloroacetate (DCA, 5 mM for 4 h) (lane 2). Primary antibody: PhosphoDetect™ Anti PDH-E1α (pSer<sup>293</sup>) Rabbit pAb (Cat. No. AP1062) PhosphoDetect™ Anti PDH-E1α (pSer<sup>300</sup>) Rabbit pAb (Cat. No. AP1064) PhosphoDetect™ Anti PDH-E1α (pSer<sup>232</sup>) Rabbit pAb (Cat. No. AP1063) all used at 0.25 µg/ml and Anti-PDHE-1α. Detection: chemiluminescence. Data courtesy of Matthew Rardin, University of California San Diego.  Detection of PDH-E1α, phosphorylated at Ser<sup>293</sup>, Ser<sup>300</sup>, and Ser<sup>232</sup> by immunocytochemistry. Samples: COS7 cells incubated in presence dichloroacetate (DCA; 5 mM for 2 h). All samples were incubated with MitoTracker<sup>®</sup> Red stain (100 nM for 20 min; to visualize mitochondria) and fixed in 3.7% formaldehyde, followed by methanol (3 min at -20°C), and permeabilization in PBS, 6% BSA, 0.3% Triton™ X-100 detergent, 0.1% Tween<sup>®</sup> 20 detergent. Primary antibodies: PhosphoDetect™ Anti-PDH-E1α (pSer<sup>293</sup>) Rabbit pAb (Cat. No. AP1062) (panel B), PhosphoDetect™ Anti-PDH-E1α (pSer<sup>300</sup>) Rabbit pAb (Cat. No. AP1064) (panel C), PhosphoDetect™ Anti-PDH-E1α (pSer<sup>232</sup>) Rabbit pAb (Cat. No. AP1063) (panel D) (0.5 µg/ml, green fluorescence) and anti-PDH-E1α antibody (green fluorescence, top 3 panels) (panel A). Detection: fluorescence (Alexa Fluor<sup>®</sup> 488 secondary antibody) with DAPI counterstain (300 nM, 1 min, blue fluorescence). Data courtesy of Matthew Rardin, University of California San Diego. |
