For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
19 June 2013
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This product has been discontinued.
| Alternatives | |
|---|---|
| 71296 | PhosphoSafe™ Extraction Reagent |
| Alternative products for PhosphoDetect™ Anti-STAT1 (pSer727) Rabbit pAb | |
| 71296 | PhosphoSafe™ Extraction Reagent |
| Accessories for PhosphoDetect™ Anti-STAT1 (pSer727) Rabbit pAb |
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Ser727 phosphorylation site of human STAT1 | ||
| Form | Liquid | ||
| Formulation | In Dulbecco's PBS, 1 mg/ml BSA, 50% glycerol, pH 7.3. | ||
| Species reactivity | human, mouse, rat | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | UV-treated HEK293 cells or LIF-treated 3T3-L1 adipocytes. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of human STAT1, phosphorylated on Ser727 by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) or treated with UV (lanes 2-5) Primary antibody: PhosphoDetect™ Anti-STAT1 (pSer727) Rabbit pAb (Cat. No. 569383) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine-containing peptide (lane 4), or the phosphopeptide immunogen (lane 5). Detection: chemiluminescence. |
