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01 June 2012
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Detects and quantifies the level of c-Kit protein that is phosphorylated at Tyr823 in human cells. c-Kit is a member of the receptor tyrosine kinase subfamily that includes PDGF, CSF-1, and FLT-3/FLK-2 receptors. It plays a critical role in controlling a variety of cellular functions in hematopoietic stem cells, mast cells, melanocytes, and germ cells.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, not mouse, not rat | ||
| Unit definition | One unit is defined as the amount of c-Kit (pTyr823) derived from approximately 40,000 Mo7e cells treated with 50 ng/ml SCF for 10 min. | ||
| Sensitivity | <1.0 Unit/ml | ||
| Assay range | 1.6 - 100 Units/ml | ||
| Assay time | 4 h | ||
| Sample type | Cell lysate | ||
| Kit contains | c-Kit (pTyr823) Standard, Standard Diluent Buffer, c-Kit Antibody-Coated 96-Well Plate, Rabbit Anti-c-Kit (pTyr823) Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
||
| Canadian export regulations | Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada. | ||
| Data | |||
|---|---|---|---|
 The sensitivity of this ELISA was compared to immunoblotting using known quantities of c-Kit (pTyr<sup>823</sup>). The data show that the sensitivity of the ELISA is approximately 2X greater than that of immunoblotting. The bands shown in the immunoblot data were developed using a rabbit anti-c-Kit (pTyr<sup>823</sup>) antibody and chemiluminescent detection. |
