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Merck KGaA
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01 June 2012
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Detects and quantifies the level of FAK (Focal Adhesion Kinase) phosphorylated at Tyr397. FAK is a non-receptor tyrosine kinase that has been implicated in several cell processes including the regulation of cell motility, cell morphology and cell adhesion.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, mouse | ||
| Unit definition | One unit is defined as the amount of FAK (pTyr397) autophosphorylated from 300 pg total FAK. | ||
| Sensitivity | < 0.9 Units/ml | ||
| Assay range | 1.6-100 Units/ml | ||
| Assay time | 4 h | ||
| Sample type | Cell lysate | ||
| Positive control | 3T3L1 cells | ||
| Kit contains | FAK (pTyr397) Standard, Standard Diluent Buffer, FAK Antibody-Coated 96-Well Plate, Rabbit Anti-FAK(pTyr397) Detector Antibody, Goat Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Canadian export regulations | Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada. | ||
| Data | |||
|---|---|---|---|
  The sensitivity of this ELISA was compared to immunoblotting using known quantities of FAK (pTyr<sup>397</sup>). The data show that the sensitivity of the ELISA is approximately 4x greater than that of immunoblotting. The bands shown in the immunoblot data were developed using anti-FAK (pTyr<sup>397</sup>) and chemiluminescent detection.  Samples were assayed 48 times times in multiple assays to determine precision between assays.  The specificity of this assay for phosphorylated FAK (Tyr<sup>397</sup>) was confirmed by peptide competition. The data show that only the phospho-peptide containing the phosphorylated (Tyr<sup>397</sup>) could block the ELISA signal. The nonphosphorylated peptide sequence or other phosphopeptides from the FAK sequence did not block the signal. |
