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71237  pIEx™-1 Ek/LIC Vector Kit

InsectDirect™ , pIEx™-1 Ek/LIC Vector Kit

For general questions please contact our Customer Service:

Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000

Contact us


01 June 2012

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Product number Qty/Pk Quantity Price
71237-3  20 rxn  loading
Prices are subject to change without notice.
The pIEx™-1 Ek/LIC Vector is designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. This vector features the hr5 enhancer and the IE1 (immediate early) promoter to direct expression in insect cells. This promoter/enhancer combination recruits endogenous insect cell transcription machinery, thereby avoiding baculovirus infection and the associated cytotoxic effects. Cloning sites are available for producing fusion proteins also containing cleavable His•Tag® and S•Tag™ sequences for detection, purification and quantification. A C-terminal HSV•Tag® sequence serves as a cloning option to permit detection of target protein with the HSV•Tag Monoclonal Antibody.
Product information
Components
1 µgEK/LIC Vector
8 µlEk/LIC b-Gal Control Insert
25 ULIC-qualified T4 DNA Polymerase
50 µlT4 DNA Polymerase Buffer
100 µl100 mM DTT
40 µl25 mM dATP
50 µl25 mM EDTA
1.5 mlNuclease-free Water
22 × 50 µlNovaBlue GigaSingles™ Competent Cells
5 × 2 mlSOC Medium
10 µlTest Plasmid
Declaration pIEx is a trademark of EMD Biosciences, Inc., Madison, WI USA
Store and ship information
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS

© Merck KGaA, Darmstadt, Germany, 2012


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