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70123  Protein Refolding Kit

Simple solubilization and refolding of inclusion body proteins

For general questions please contact our Customer Service:

Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000

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01 June 2012

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Product number Qty/Pk Quantity Price
70123-3  1 kit  loading
Prices are subject to change without notice.
The Protein Refolding Kit provides the basic reagents to solubilize and refold recombinant proteins that accumulate as inclusion bodies in E. coli by a patented procedure (Burgess 1996, Frankel 1993).
Product information
Avoid freeze/thaw Yes
Components
100 ml10X IB Wash Buffer
50 ml10X IB Solubilization Buffer
2 x 100 ml50X Dialysis Buffer
2 x 1.6 ml1M DTT
10 ml30% N-lauroylsarcosine
Features and benefits
  • Provides for easy isolation and solubilization of inclusion bodies under mild denaturing conditions.
  • Solubilized fraction is dialyzed against a neutral pH buffer containing a reducing agent to encourage correct disulfide bond formation.
  • A second dialysis step removes excess reducing agent and transfers the protein into the buffer of choice
  • Additional separate components such as NDSBs can be added to increase refolding efficiency of individual proteins.
  • Sufficient buffers are provided to process up to 2 g inclusion bodies.
Store and ship information
Storage +2°C to +8°C
Ship Blue Ice Only
Multiple Toxicity Values, refer to MSDS

© Merck KGaA, Darmstadt, Germany, 2012


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