71532  ProteoEnrich™ CAT-X Kit

Highly specific, reproducible, convenient methods to reduce proteome complexity

The ProteoEnrich™ CAT-X Kit provides a convenient and reproducible method to fractionate the proteome of a given biological sample under non-denaturing conditions prior to downstream analysis by one- and two-dimensional gel electrophoresis, liquid chromatography, protein arrays and functional tests such as activity assays and ELISA. The ProteoEnrich CAT-X Kit procedure fractionates proteins based on their binding to the strong cation exchange resin, Fractogel^® EMD SO₃. Nearly the complete proteome binds to the matrix under slightly acidic conditions, and partial proteomes can be eluted with a salt gradient. Excess salt should be removed prior to 2D gel electrophoresis. The kit contains two CAT-X cartridges with Luer Lock Adapters, and optimized buffers. The CAT-X cartridges can be used as syringe-tip filters or as liquid chromatography devices. Each cartridge binds up to 140 mg of a single protein or up to 5 mg of a complex protein mixture and can be reused at least 10 times.