71539  ProteoEnrich™ CAT-X SEC Kit

Highly specific, reproducible, convenient methods to reduce proteome complexity

The ProteoEnrich CAT-X SEC Kit provides a highly specific multidimensional chromatography method to enrich the proteome of a biological sample such as body fluids and crude tissue extracts for low molecular weight proteins. The method is based on a unique resin that allows proteins and polypeptides with a globular size of less than 20 kDa to penetrate the resin pores and bind to the surface of the inner pores according to their net charge while larger proteins flow through the resin-filled cartridge. After a salt gradient or single step elution and excess salt removal, the sample is ready for downstream analysis by mass spectrometry, SELDI, or array-based analysis. The kit contains two CAT-X SEC cartridges with Luer Lock Adaptors and optimized buffers. The cartridges can be used as syringetip filters or as liquid chromatography devices. Each cartridge binds up to 10 mg complex protein mixture and can be reused at least 10 times.