Wenn Sie allgemeine Fragen haben, wenden Sie sich bitte an unseren Kundenservice:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Telefon: +49 6151 72-0
Fax: +49 6151 72 2000
01 Juni 2012
wird geladen
This kit provides a unique combination of reagents useful for the isolation of a highly enriched mitochondrial fraction from the cytosol. The enriched fractions can be used to study factors of interest using Western blotting, ELISA, or other assays.
Note: 1 Test = reagents sufficient for processing 5 x 107 cells.
| Produktinformationen | |||
|---|---|---|---|
| Format | Cell extraction | ||
| Form | 100 Extractions | ||
| Avoid freeze/thaw | Ja | ||
| Sample type | Cells | ||
| Kit contains | Mitochondria Extraction Buffer, Cytosol Extraction Buffer, Protease Inhibitor Cocktail, Reducing Reagent, and a user protocol. | ||
| Lager- und Versandinformationen | |||
|---|---|---|---|
|
Achtung Gefahrgut:Aufgrund der Natur des in dieser Lieferung enthaltenen Gefahrgutes können zusätzliche Versandkosten für Ihren Auftrag entstehen. Bestimmte Größen können ggf. von den Versandzuschlägen für Gefahrgüter ausgenommen sein. Bitte kontaktieren Sie Ihr lokales Verkaufsbüro für weitere Informationen bzgl. dieser Gebühren. |
|||
| Lagerkategorie | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
||
| Sicherheitshinweise | |||
|---|---|---|---|
| S-Satz |
S: 23-26-36/37/39-45 |
||
| R-Satz |
R: 23/24/25-32-34-36/37/38-41 |
||
| Daten | |||
|---|---|---|---|
 Mitochondrial and cytosolic fractions were isolated from HEPG2 cells using QIA88 ProteoExtract® Cytosol/ Mitochondria Fractionation Kit. Mitochondrial pellets were resuspended in PBS. The protein concentration of each fraction was determined using the BCA assay. DTT was omitted from the Cytosol Extraction Buffer as it can interfere with concentration determination in the BCA assay. Equivalent amounts (10 µg) of HEPG2 homogenate (total protein), cytosolic, and mitochondrial fractions were analyzed by Western blot for proteins localized to different cellular regions as indicated. Representative results from 2 independent fractionations are shown. |
